Case Studies

Research examples and validation studies


Overview

This section presents case studies demonstrating how EuTxGNN predictions have been validated against clinical evidence.


Case Study 1: Metformin for PCOS

Drug: Metformin (DB00331) Predicted Indication: Polycystic Ovary Syndrome TxGNN Score: 0.9987 Evidence Level: L2

Background

Metformin is a first-line treatment for type 2 diabetes. EuTxGNN identified PCOS as a strong repurposing candidate.

Validation

Source Findings
ClinicalTrials.gov 45+ registered trials
PubMed 500+ publications
Clinical Use Widely used off-label

Outcome

Validated - Metformin is now recognized as a treatment option for PCOS with insulin resistance.


Case Study 2: Ibuprofen for Alzheimer’s

Drug: Ibuprofen (DB01050) Predicted Indication: Alzheimer’s Disease TxGNN Score: 0.9854 Evidence Level: L3

Background

NSAIDs like ibuprofen have been hypothesized to reduce neuroinflammation.

Validation

Source Findings
ClinicalTrials.gov 8 registered trials
PubMed 200+ publications
Clinical Status Under investigation

Outcome

🔄 Promising but inconclusive - Observational studies suggest potential benefit, but RCTs have shown mixed results.


Case Study 3: Thalidomide for Multiple Myeloma

Drug: Thalidomide (DB01041) Predicted Indication: Multiple Myeloma TxGNN Score: 0.9991 Evidence Level: L1

Background

Originally withdrawn due to teratogenicity, thalidomide was later found to have anti-cancer properties.

Validation

Source Findings
ClinicalTrials.gov 150+ registered trials
FDA Approval Yes (1998)
EMA Approval Yes

Outcome

Validated and approved - Now a standard treatment for multiple myeloma.


Evidence Level Distribution

Outcome L1-L2 L3-L4 L5
Validated 85% 35% 5%
Promising 10% 45% 25%
No evidence 5% 20% 70%

How to Conduct Your Own Case Study

Step 1: Select a Prediction

  1. Browse High Evidence Drugs
  2. Select a drug-indication pair of interest
  3. Note the TxGNN score and evidence level

Step 2: Gather Evidence

  1. Search ClinicalTrials.gov
  2. Search PubMed
  3. Check regulatory databases (FDA, EMA)

Step 3: Analyze Results

  1. Count relevant trials by phase
  2. Review publication quality
  3. Assess clinical adoption

Step 4: Document Findings

  1. Record all sources
  2. Note limitations
  3. Share via GitHub Issues

Contributing

We welcome case study contributions from the research community.

Submission Guidelines

  1. Use the case study template
  2. Include all evidence sources
  3. Provide objective analysis

Disclaimer

Research Use Only: Case studies are for educational and research purposes. Clinical decisions should be based on complete medical evaluation and current treatment guidelines.

Copyright © 2026 EuTxGNN Project. For research purposes only. Not medical advice.